Quantification of Short-Chain Fatty Acids in Cecal Material by Gas Chromatography-Mass Spectrometry
نویسنده
چکیده
..................................................................................................................................................v INTRODUCTION....................................................................................................................................1 1. Gastrointestinal tract (GI)............................................................................................................1 2. Microbiota in Humans..................................................................................................................2 3. Health benefits of short chained fatty acids..............................................................................3 4. Sources of SCFA production.........................................................................................................5 5. Analysis of SCFA of gas chromatography/mass spectrometry................................................7 6. Gas chromatography mass spectrometric detection of analytes...........................................8 7. Use of mouse model system........................................................................................................9 EXPERIMENTAL PROCEDURES............................................................................................................10 1. Preparation of standards............................................................................................................10 2. Preparation of pentaflouro benzyl bromide esters of fatty acids........................................10 3. Hydroxyl fatty acid analysis.......................................................................................................11 4. Conversion of hydroxyl fatty acids to their (bis)-TMS ester derivatives by use of BSTFA...........................................................................................................................11
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